Journal
MICROCHIMICA ACTA
Volume 182, Issue 5-6, Pages 995-1001Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-014-1420-5
Keywords
Aflatoxin B2; Aptamer; Gold nanoparticles; Colorimetry; Mycotoxin
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Funding
- National Science Foundation of China [41,301,350]
- Beijing Academy of Agriculture and Forestry Sciences [KJCX20140302]
- Beijing Research Center for Agricultural Standards and Testing [ATFM-KFKT2013003]
- National High Technology Research and Development Program of China (863 Program) [2012AA101609]
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We describe a fast and sensitive method for the detection of aflatoxin B2 (AFB2) that is based on the addition of AFB2-sensitive aptamers to a colloidal solution of gold nanoparticles (AuNPs). Subsequent addition of AFB2 and NaCl to the solution causes a color change from wine-red to blue-purple. Best results are obtained at a pH value of 7.0, a 10 mM aptamer concentration, and a 2 M concentration of NaCl. The method has a linear dynamic range that extends from 0.025 to 10 nga (TM) mL(-1) of AFB2, and the detection limit is 25 pga (TM) mL(-1). The method is simple, fast, highly sensitive, and enables both visual and microplate readout.
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