Journal
MICROCHIMICA ACTA
Volume 181, Issue 5-6, Pages 519-525Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-013-1143-z
Keywords
Blood glucose; Biosensor; Rh nanoparticle; Gold nanoparticle; Nafion
Categories
Funding
- National Natural Science Foundation of China [30600143, 81171416]
- National Key Technology R&D Program of China [2012BAI16B02]
- Zhejiang Provincial Natural Science Foundation of China [Y13H180019]
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We have developed an enzymatic glucose biosensor that is based on a flat platinum electrode which was covered with electrophoretically deposited rhodium (Rh) nanoparticles and then sintered to form a large surface area. The biosensor was obtained by depositing glucose oxidase (GOx), Nafion, and gold nanoparticles (AuNPs) on the Rh electrode. The electrical potential and the fractions of Nafion and GOx were optimized. The resulting biosensor has a very high sensitivity (68.1 mu A mM(-1) cm(-2)) and good linearity in the range from 0.05 to 15 mM (r = 0.989). The limit of detection is as low as 0.03 mM (at an SNR of 3). The glucose biosensor also is quite selective and is not interfered by electroactive substances including ascorbic acid, uric acid and acetaminophen. The lifespan is up to 90 days. It was applied to the determination of glucose in blood serum, and the results compare very well with those obtained with a clinical analyzer.
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