Journal
MICROCHIMICA ACTA
Volume 181, Issue 9-10, Pages 941-948Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-014-1192-y
Keywords
Carcinoma marker; Magnetic nanoparticles; beta-cyclodextrin; Solid-phase extraction
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Funding
- Science and Innovation Spanish Ministry [MICINN-09-CTQ2009-09595, MAT2012-099]
- Erasmus Mundus Medastar grant
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We describe the synthesis of -cyclodextrin modified magnetic nanoparticles (CD-mNPs) as a material for solid-phase extraction of the cancer biomarker 5-hydroxy-indole-3-acetic acid (5-HIAA) from urine. The CD-mNPs were characterized by TEM, FTIR, and XRD, and the kinetics and adsorption isotherms were studied. The strong interaction between the CD-mNPs and 5-HIAA is the main driving force for recognition and extraction, while the magnetic core of the NPs allows their separation from the sample matrix. Recovery of 5-HIAA from the adsorbent using an adequate solvent regenerated the adsorbent for further use. 5-HIAA was then quantified by fluorometry of its complex with -CD. The method works in the 1 Au 10(-7) to 1 Au 10(-5) mol L-1 (R-2 0.9982-0.9996) concentration range, and the limits of detection (3 sigma) and quantification (10 sigma) of the method are 1.2 Au 10(-8) mol L-1 and 4.01 Au 10(-8) mol L-1 5-HIAA, respectively. The recovery of 5-HIAA from urine samples spiked with 5-HIAA in three concentrations (1.4 Au 10(-6), 4.50 Au 10(-6) and 1.0 Au 10(-5) mol L-1) are within 63 +/- 3 %.
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