Journal
MICROCHIMICA ACTA
Volume 182, Issue 3-4, Pages 643-651Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-014-1370-y
Keywords
Organophosphate; Butyrylcholinesterase; Screen-printed electrode; Carbon black nanoparticles; Inhibition
Categories
Funding
- National Industria [MI01_00223]
- ACQUA-SENSE project
- Marie Curie FP7-PEOPLE-2011-IRSES [294901]
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We have developed a screen-printed electrochemical electrode (SPE) for paraoxon based on its inhibitory effect on the enzyme butyrylcholinesterase (BChE). The electrode was first modified by drop casting with a dispersion of carbon black nanoparticles (CBNPs) in a dimethylformamide-water mixture, and BChE was then immobilized on the surface by cross-linking. The resulting biosensor was exposed to standard solutions of paraoxon, and the enzymatic hydrolysis of butyrylthiocholine over time was determined measuring the enzymatic product thiocholine at a working voltage of +300 mV. The enzyme inhibition is linearly related to the concentration of paraoxon up to 30 mu g L-1, and the detection limit is 5 mu g L-1. The biosensor is stable for up to 78 days of storage at room temperature under dry conditions. It was applied to determined paraoxon in spiked waste water samples. The results underpin the potential of the use of CBNPs in electrochemical biosensors and also demonstrate that they represent a viable alternative to other carbon nanomaterials such as carbon nanotubes or graphene, and with the advantage of being very affordable.
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