Journal
MICROCHIMICA ACTA
Volume 182, Issue 1-2, Pages 331-339Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-014-1338-y
Keywords
SELEX; Magnetic microparticle, Aptamers, Brain natriuretic peptide (BNP), Rapid and early diagnosis
Categories
Funding
- Huangshan Young Scholar Fund of Hefei University of Technology [407-037025]
- NSFC [31328009, 31301460]
- Science and Technology Research Project of General AQSIQ of China [201210127, 201310135]
- 12th 5 Years Key Programs [2012BAK08B01-2, 2012BAK17B10, SS2012AA101001]
- National and Zhejiang Public Benefit Research Project [201313010, 2014C32051]
- Fundamental Research Funds for the Central Universities [2013HGCH0008]
- [62YYD201309]
- [2012BAD43B03]
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The brain natriuretic peptide (BNP) is known to be an effective indicator of heart failure. It has been widely adopted as a parameter for the evaluation of heart function of cardiovascular and cerebrovascular diseases (CVDs). Current immune-recognition based methods for the detection of BNP are limited, to a certain extent, by the poor stability of the antibody and by high costs. The availability of an aptamer specific for BNP would greatly assist in the rapid and early diagnosis of CVDs. In order to screen for such an aptamer by the SELEX method, we have used magnetic microparticles (m-MPs) as the separation substrate for immobilization of target BNP. The use of m-MPs for rapid separation of combined aptamers enables bound oligonucleotides to be separated directly, quickly, and with high efficiency. After 14 rounds of selection, a panel of six aptamers against BNP was identified. Their dissociation constants range from 12.5 to 139 nM. The classical technique for conjugation of a target to m-MPs is known to be applicable to various fields, and we conclude that this m-MP-based SELEX process provides a general strategy for screening of specific aptamers against various analytes.
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