Journal
MICROCHIMICA ACTA
Volume 180, Issue 11-12, Pages 1165-1172Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-013-1043-2
Keywords
Lateral flow; Microarray; Multiplex immunoassay; Drugs of abuse
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Funding
- Ministry of Education and Science of the Russian Federation [16.512.11.2125]
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We report on a lateral flow microarray that combines multi-spot immunochip technology and immunochromatography. It can serve as a tool for the simultaneous detection of multiple analytes. The test zone of the nitrocellulose support comprises a microarray spotted with up to 32 antigens that can capture labeled gold-antibodies after lateral flow. The detection limits and detectable concentration ranges of the assay were characterized. The method was applied to the determination of drugs of abuse (and their metabolites) in urine, specifically of morphine, amphetamine, methamphetamine, and benzoylecgonine. The assay format is rapid (10 min), and has both a low relative standard deviation (< 9 %) and high recoveries (95-114 %). The detection limits (2-20 ng mL(-1) for drugs of abuse) are comparable to those of conventional single-analyte strip methods.
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