Journal
MICROCHIMICA ACTA
Volume 179, Issue 3-4, Pages 201-207Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-012-0880-8
Keywords
Graphene oxide; HER2; ssDNA-labeled; SKOV-3; Immunosensor
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Funding
- Foundation of Shanghai Municipal Government [08520510400]
- Shanghai Leading Academic Discipline Project [S30406]
- Key Laboratory of Resource Chemistry of Ministry of Education
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We describe a highly sensitive electrochemical immunoassay for the tumor maker HER2 on the surface of SKOV-3 human ovarian cancer cells. Following the binding of the cancer cells, ssDNA-labeled anti-HER2 antibody (ssDNA-Ab; the detection antibody) was added to conjugate unbound antigen on the target cells. Following hybridization of ssDNA with its complementary DNA, daunorubicin was injected in order to intercalate into the duplex. This enables electron transfer between daunorubicin and electrode to take place. The GO film strongly amplifies the redox signal of daunorubicin. This new assay has a detection limit of 5.2 cells per mL and in our opinion holds great promise for clinical screening of cancer biomarkers and point-of-care diagnostics.
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