Journal
MICROCHIMICA ACTA
Volume 178, Issue 3-4, Pages 349-355Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-012-0850-1
Keywords
Aptamer; Thrombin; Magnetic beads; Quantum dots
Categories
Funding
- National Natural Science Foundation of China [20905043]
- Shanxi Scholarship Council of China
- State Key Laboratory of Environmental Chemistry and Ecotoxicology in the Research Center for Eco-Environmental Sciences
- Chinese Academy of Sciences [KF2010-24]
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We describe an aptamer-based sandwich assay for thrombin by using a pair of thrombin-binding aptamers, namely one 15-mer aptamer (denoted as Apt15) and one 29-mer aptamer (denoted as Apt29). Either Apt29 or Apt15 can be used as capture aptamers on magnetic beads or reporter aptamers on the quantum dots to form the sandwich complex. Detection of thrombin is achieved by the fluorescent measurement of quantum dots in the sandwich complex. The choice of capture aptamers and reporter aptamers, and the effect of the addition order of the aptamers modified magnetic beads and the aptamers modified quantum dots were investigated. Detection of 0.05 nM thrombin was accomplished. The proteins hemoglobin, lysozyme, and transferrin did not interfere in this assay.
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