Journal
MICROCHIMICA ACTA
Volume 176, Issue 3-4, Pages 327-335Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-011-0719-8
Keywords
High-performance liquid chromatography-fluorescence detection; Graphene-based solid-phase extraction; Neurotransmitter amino acids; Rat brain samples
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Funding
- National Natural Science Foundation of China [20805040]
- Program for Science & Technology Innovation Talents in Universities of Henan Province [2010HASTIT025]
- Excellent Youth Foundation of He'nan Scientific Committee [104100510020]
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A simple, cost-effective and efficient method was developed for the determination of glycine, gamma-aminobutyrate and taurine in rat brain using graphene as a sorbent for solid-phase extraction. The analytes were eluted from a graphene-packed solid-phase extraction cartridge with methanol, derivatized at their amino groups with the fluorescent label 4-carboxy-2,6-dimethylquinoline N-hydroxysuccinimide ester, and then separated and fluorescently detected by HPLC. The type and volume of eluent, sample pH, extraction time and sample volume were optimized with respect to sensitivity and precision. Under optimal conditions, linear response is obtained in the concentration range from 0.1 to 50 mu g g-1, with correlation coefficients of > 0.990. The limits of detection are 23.4 ng g(-1) (gamma-aminobutyrate), 45.3 ng g(-1) (glycine) and 67.5 ng g(-1) (taurine) (S/N = 3). The results reveal the potential of graphene as a sorbent in the analysis of biological samples.
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