Journal
MICROCHIMICA ACTA
Volume 168, Issue 3-4, Pages 221-229Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-009-0270-z
Keywords
Biosensor; Biofuel cell; Graphite rod electrode; Electron transfer; Gold nanoparticles; Glucose oxidase; Amperometric sensors
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Funding
- Lithuanian State Science and Studies Foundation [S-6/2007]
- Agency for International Science and Technology Development Programmes in Lithuania
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The electrochemistry of glucose oxidase (GOx) immobilized on a graphite rod electrode modified by gold nanoparticles (Au-NPs) was studied. Two types of amperometric glucose sensors based on GOx immobilized and Au-NPs modified working electrode (Au-NPs/GOx/graphite and GOx/Au-NPs/graphite) were designed and tested in the presence and the absence of N-methylphenazonium methyl sulphate in different buffers. Results were compared to those obtained with similar electrodes not containing Au-NPs (GOx/graphite). This study shows that the application of Au-NPs increases the rate of mediated electron transfer. Major analytical characteristics of the amperometric biosensor based on GOx and 13 nm diameter Au-NPs were determined. The analytical signal was linearly related to glucose concentration in the range from 0.1 to 10 mmol L-1. The detection limit for glucose was found within 0.1 mmol L-1 and 0.08 mmol L-1 and the relative standard deviation in the range of 0.1-100 mol L-1 was 0.04-0.39%. The tau(1/2) of V (max) characterizes the storage stability of sensors: this parameter for the developed GOx/graphite electrode was 49.3 days and for GOx/Au-NPs/graphite electrode was 19.5 days. The sensor might be suitable for determination of glucose in beverages and/or in food.
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