Journal
MICROCHIMICA ACTA
Volume 172, Issue 1-2, Pages 39-49Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-010-0454-6
Keywords
Antibiotic; Residues; Carrier-mediated; Hollow fiber; Liquid-phase microextraction; Sample preparation
Categories
Funding
- Chulalongkorn University, Ratchadaphiseksomphot Endowment
- Thailand Research Fund
- Commission on Higher Education
- Research Grant for Mid-Career University Faculty [RMU 5180009]
- comprehensive centre for innovation on food, health products and agriculture [TKK 2555]
- national research university
- Center for Petroleum, Petrochemicals, and Advanced Materials at Chulalongkorn University
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A method has been developed for carrier-mediated hollow-fiber liquid-phase microextraction (HF-LPME) and enrichment of multiple classes of antibiotics in water samples. Eleven compounds (erythromycin, spiramycin, tilmicosin, sulfathiazole, sulfamethazine, sulfamerazine, oxytetracycline, tetracycline, ciprofloxacin, danofloxacin and enrofloxacin) from four important classes of antibiotics (of the macrolide, sulfonamide, tetracycline and quinolone type) have been simultaneously preconcentrated with one set of HF-LPME conditions, followed by determination by ultra-HPLC combined with electrospray ionization tandem mass spectrometry (UHPLC-MS/MS). Antibiotics can be determined at ng L-1 levels using this highly sensitive and selective method. Parameters including immersion time, liquid membrane composition, sample pH, acceptor composition and extraction time were optimized to finally give detection limits in the 10-250 ng L-1 range. Good linearity was achieved, with up to 156 times enrichment over the four classes of antibiotics. This multi-residue method enabled the simultaneous enrichment of all 11 multi-class antibiotics from spiked river water samples, with relative recovery between 79 and 118%.
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