Amperometric glucose biosensor based on platinum nanoparticle encapsulated with a clay

A hybrid system was constructed in neutral aqueous solution that consisted of clay clusters incorporated with platinum nanoparticles that were encapsulated with a poly(amidoamine) dendrimer through electrostatic interaction. Glucose oxidase was immobilized in this hybrid and used to modify a glassy carbon electrode so to obtain an enzymatic glucose biosensor. The nanohybrid material and the sensor were characterized by cyclic voltammetry and amperometry. The sensor has a response time of <3 s and a dynamic range from 0.01 to 16 mM. The detection limit is 4 mu M at a signal-to-noise ratio of 3.