Journal
MICROCHIMICA ACTA
Volume 170, Issue 3-4, Pages 275-281Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-010-0358-5
Keywords
Nanoelectrodes; Interdigitated electrodes; Impedimetric genosensor; Gold nanoparticle amplification; BRCA1 gene
Categories
Funding
- Ministry of Education and Science (MEC, Madrid, Spain) [CSD2006-00012]
- Generalitat de Catalunya
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A DNA biosensor is presented that is based on gold interdigitated nanoelectrodes of 100 nm width and 250 nm pitch. A single-strand oligonucleotide acts as the capture probe. Because of its nanometer dimensions, the device shows improved sensitivity when compared to similar systems. This encouraged us to perform a direct and unlabelled detection. After incubation with the DNA target, the impedance spectrum was recorded between 1 kHz and 10 MHz to obtain the net capacitance change. The use of a biotinylated DNA signalling probe permitted the integration of an amplification stage in a sandwich format that employs streptavidin-modified gold nanoparticles. The strategy was preliminarily tested by detecting the breast cancer related BRCA1 gene, where the noncomplementary, wild and mutated forms were easily differentiated at a concentration level of 3 mu M (corresponding to a 30 pmol quantity).
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