Preparation of a novel molecularly imprinted polymer by a sol-gel process for on-line solid-phase extraction coupled with high performance liquid chromatography to detect trace enrofloxacin in fish and chicken samples

A molecularly imprinted functionalized sol-gel has been prepared by using enrofloxacin (ENRO) as a template, 3-aminopropyltriethoxysilane as the functional monomer, tetraethoxysilicane as a cross-linker. This ENRO-imprinted polymer was evaluated by static, kinetic adsorption and selective experiments. The polymer displays good selectivity, and fast kinetics in terms of adsorption and desorption. The material was applied as a sorbent for on-line determination of trace ENRO by high performance liquid chromatography, with a focus on biological matrices. At a loading flow rate of 1.0 mL min(-1) for sampling 50 mL, the enrichment factor and limit of detection (LOD; at S/N = 3) are 566 and 8 ng L-1, respectively. The peak area precision (the LOD for nine replicate detections of 0.1 A mu g L-1 ENRO) is 4.4%. ENRO in fish and chicken muscle was determined at three spiked levels with recoveries ranging from 70% to 82%.