Journal
MICROCHIMICA ACTA
Volume 166, Issue 1-2, Pages 21-26Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-009-0154-2
Keywords
Direct electrochemistry; DNA biosensor; MWNTs modified electrode; Determination of phenols
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Funding
- Huazhong University of Science and Technology [2006Z002A]
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A DNA biosensor was constructed by immobilizing DNA on a glassy carbon (GC) electrode modified with multiwall carbon nanotubes (MWNTs) dispersed in Nafion (DNA/MWNTs/GCE). The DNA-modified electrode exhibited two well-defined oxidation peaks corresponding to the guanine and adenine residues of DNA, respectively. The effects of the adsorption potential, DNA concentration and quantity of MWNTs used for DNA immobilization were investigated, as were the effects of buffer, pH and scan rate on the voltammetric behavior of DNA. Phenol, m-cresol and catechol showed noticeable inhibition towards the response of the electrode due to their interactions with DNA. These findings were used to design biosensors with linear response to these phenolic pollutants.
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