Journal
MICROCHIMICA ACTA
Volume 166, Issue 3-4, Pages 283-288Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-009-0202-y
Keywords
Gold nanoparticles; HRP; PDDA; Self-assembly; Direct electrochemistry
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Funding
- Opening Foundation of State Key Laboratory for Chemo/Biosensing and Chemometrics [2006012]
- Shanghai Key Laboratory Project [07DZ22303]
- Foundation of Shanghai Normal University [DYL200703, DYL701]
- Leading Academic Discipline Project of Shanghai Normal University [DZL706]
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Poly(diallyldimethylammonium chloride), gold nanoparticles (Au-NPs; 2-5 nm) and horseradish peroxidase (HRP) were alternately self-assembled on a glassy carbon electrode. Due to the conducting wire effect of the Au-NPs, the HRP undergoes direct electrochemistry with a small peak-to-peak separation of 33 mV and a formal potential of -0.370 V (versus SCE). The reaction involves a single electron transfer coupled to a one-proton transfer reaction. Electrochemical impedance spectroscopy and cyclic voltammetry also were applied to characterize the self-assembly process and to study the electrochemical behavior of the immobilized HRP. Its good electrocatalytic response to the reduction of hydrogen peroxide resulted in a novel third-generation biosensor for this species.
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