Journal
MICROCHIMICA ACTA
Volume 165, Issue 3-4, Pages 437-442Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-009-0159-x
Keywords
Magnetic nanorods; Carcinoembryonic antigen; Potentiometric immunosensor; Label-free
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Funding
- Scientific Research Fund of Sichuan Provincial Education Department [08zb001]
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A new potentiometric immunoassay protocol for the determination of carcinoembryonic antigen (CEA) in human serum was designed by using a novel transparent immunoaffinity reactor. To construct such an affinity reactor, Fe3O4 nanorods with average diameter of 40 nm and length of 1.0 mu m were initially synthesized through hydrolysis of FeCl3 and FeSO4 in the urea solution, and then the synthesized nanorods were used as the immobilized affinity support for the conjugation of anti-CEA antibodies (bionanorods). With an external magnet, the magnetic bionanorods were attached to the surface of a self-made carbon paste electrode. The performance and factors influencing the immunosensors were evaluated. Under optimal conditions, the binding of CEA in the samples onto the immobilized anti-CEA changed the potential response, a dynamic range from 1.5 to 80 ng center dot mL(-1), and a detection limit of 0.9 ng center dot mL(-1) CEA (at 3 sigma). The immunosensors show good precision, high sensitivity, acceptable stability and reproducibility. The CEA concentrations of the clinical serum specimens assayed by the immunosensor show consistent results in comparison with those obtained by commercially available enzyme-linked immunosorbent assay.
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