Journal
MICROCHIMICA ACTA
Volume 165, Issue 3-4, Pages 285-290Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-008-0130-2
Keywords
Transferrin; Albumin; Micellar electrokinetic chromatography; Radioimmunoassay; Human urine
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Funding
- Education Committee of Hubei Province [Q200722004, Z200622001]
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A micellar electrokinetic chromatography (MEKC) method for simultaneous determination of urinary transferrin and albumin has been developed. The performance of the assay was evaluated. Under the optimum conditions, the linearity of transferrin and albumin is in the range of 10 similar to 1000 mg L-1. The detection limits are 1.3 mg L-1 for transferrin and 1.1 mg L-1 for albumin, with relative standard deviations of less than 5.4%. The within-run and between-run coefficients of variation were < 5.5% for transferrin and < 8.2% for albumin, respectively. The recoveries of transferrin and albumin are 97.7 similar to 106.1%. The performance of the MEKC assay was compared with radioimmunoassay, and correlated well with this.
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