Sensitive chemiluminescence method for the determination of glutathione, L-cysteine and 6-mercaptopurine

Glutathione (GSH), L-cysteine (L-Cys) and 6-mercaptopurine (6-MP) inhibit the CL reaction of luminol-H2O2 catalyzed by gold colloids. In order to explore this, GSH, L-Cys and 6-MP were injected into the chemiluminescence system of luminol and H2O2 catalyzed by gold colloids. The results showed that gold colloids interact with GSH, L-Cys and 6-MP and decrease the CL emission. Based on this phenomenon, a simple, sensitive and convenient flow injection CL method was developed for the determination of GSH, L-Cys and 6-MP. This method provides a novel, and effective CL assay for GSH, L-Cys and 6-MP that has been applied to the determination of GSH in human serum.