Journal
MICROCHIMICA ACTA
Volume 164, Issue 1-2, Pages 41-47Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-008-0029-y
Keywords
Hemoglobin; Silver nanoparticle; Co-immobilization; Hydrogen peroxide; Direct electrochemistry
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Funding
- National Natural Science Foundation of China [20675042]
- Social Development Item of Jiangsu Province [BS2005030]
- Jiangsu Province Education Commission Natural Science Foundation [JH03-041, 03KJB150107]
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Hemoglobin (Hb) and silver-silver oxide (Ag-Ag2O) nanoparticles were co-immobilized on a bare silver electrode surface by cyclic voltammetry, and were characterized by UV-vis reflection spectroscopy, scanning electron microscopy, and electrochemical impedance spectroscopy. The immobilized Hb was shown to maintain its biological activity well. Direct electron transfer between Hb and the resulting electrode was achieved without the aid of any electron mediator. The reduction currents to hydrogen peroxide (H2O2) at co-immobilized electrodes showed a linear relationship with H2O2 concentration over a concentration range from 6.0 x 10(-6) to 5.0 x 10(-2) mol L-1, and a detection limit of 2.0 x 10(-6) mol L-1 (S/N = 3).
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