Journal
MICROCHEMICAL JOURNAL
Volume 99, Issue 2, Pages 180-185Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.microc.2011.05.001
Keywords
Chip; Poly(dimethylsiloxane); Pressure injection; Chip electrophoresis
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Funding
- National Scientific Research Fund, Hungary [K75286]
- TAMOP [4.2.1./B-09/1/KONV-2010-0007]
- National Science Foundation [OISE-0754138]
- Office Of Internatl Science &Engineering
- Office Of The Director [0754138] Funding Source: National Science Foundation
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The ability to accurately inject small volumes of sample into microfluidic channels is of great importance in electrophoretic separations. While electrokinetic injection of nanoliter scale volumes is commonly utilized in microchip capillary electrophoresis (MCE), mobility and matrix bias makes quantitation difficult. Herein, we describe a new injection method based on the simple patterning of the crossing of channels that does not require sophisticated instrumentation. The sample volume injected into the separation channel is dependent on the ratio of the widths of the crossing channels. This injection method is capable of introducing, into a separation channel, multiple plugs of sample on a large scale. This injection technique is tested for zone electrophoresis in native and surface modified poly(dimethylsiloxane) (PDMS) chips. (C) 2011 Elsevier B.V. All rights reserved.
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