Journal
MICROCHEMICAL JOURNAL
Volume 99, Issue 1, Pages 152-158Publisher
ELSEVIER
DOI: 10.1016/j.microc.2011.04.015
Keywords
Ion exchange chromatography; 1-(2-pyridylazo)-2-naphthol; Post-column derivatization; Metal ions; Environmental samples
Categories
Funding
- Department of Chemistry, Faculty of Science, Khon Kaen University
- Environmental Engineering Department, Faculty of Engineering, Khon Kaen University
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Ion exchange chromatography (IEC) using a bi-functional column (quaternary ammonium and sulfonate groups), followed by post-column reaction (PCR) with 1-(2-pyridylazo)-2-naphthol (PAN), was used to separate and quantitate Cu(II), Ni(II), Zn(II), Co(II), Cd(II), Mn(II) and Hg(II) at low concentration levels. IEC-PCR separation was achieved within 14 min using the mobile phase containing 3 mmol L-1 2,6-pyridinedicarboxylic acid (PDCA) and 3 mmol L-1 oxalate at pH 12.5. Effects of pH as well as PAN, detergent and chloride ion concentrations during post-column reaction on detector response were examined. Detection limits were less than 4.5 mu g L-1 for all metals except Hg(II) (19 mu g L-1) using spectrophotometric measurements at 550 nm. Analytical validations showed good linearity for detection up to 6.0 mg L-1, with R-2 higher than 0.99. Precisions based on retention time evaluation for intra-day and inter-day measurements with the relative standard deviation (RSD) were less than 2.9% and 3.6%, respectively. The method gave good accuracy with the recoveries ranged from 80.5 to 105% for all metal ions studied. The proposed method was applied to the analysis of metal ions in environmental samples (leachate, soil and sediment) in Northeastern Thailand. The results were in good agreement with atomic spectroscopic measurements on the same samples. (C) 2011 Elsevier B.V. All rights reserved.
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