Journal
MICROCHEMICAL JOURNAL
Volume 94, Issue 2, Pages 125-129Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.microc.2009.10.001
Keywords
Arsenic speciation; Arsenic uptake; HPLC-ICP-MS; White mustard
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Funding
- Polish Ministry of Science and Higher Education [N204 009935]
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The concentration and speciation of arsenic incorporated by plants grown in the presence of different arsenic compounds was compared, and the influence of plant sample pretreatment and extraction procedures on the recovery and reliability of speciation analyses was studied. It was concluded that sample pretreatment greatly affected the extraction efficiency, but did not change arsenic speciation. The most suitable extraction procedure involved dried plant material without the use of liquid nitrogen. To assess the ability of White mustard to uptake arsenic in different forms, samples were cultivated in nutrient solutions containing either As(III), As(V), monomethylarsonic acid (MMA) or dimethylarsinic acid (DMA). The translocation factor was the highest (0.70) when DMA was added to the nutrient solution, however the overall As concentration in plant tissues was the lowest in this case. Only inorganic As was found in plant tissues when either As(III) or As(V) was added to the nutrient solution. When organic arsenic was present in the nutrient medium, however, it was partially transformed by the plants into inorganic forms. (C) 2009 Elsevier B.V. All rights reserved.
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