Journal
MICROCHEMICAL JOURNAL
Volume 95, Issue 2, Pages 377-387Publisher
ELSEVIER
DOI: 10.1016/j.microc.2010.02.021
Keywords
Lime wood; Biodegradation; FT-IR spectroscopy; 2D IR correlation spectroscopy
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The action of soft-rot fungus Chaetomium globosum has been studied The decayed lime wood samples were observed for different periods of exposure The degree of decay was determined by weight loss which was of 504% after 133 days The samples were analyzed by FT-IR and 2D IR correlation spectroscopy The intensity bands assigned to different vibrations from cellulose and hemicelluloses show a decrease, while the intensities of the bands assigned to C-O vibrations due to the formation of oxidized structures increase At the same time, the intensity of the band assigned to C-O in metoxyl groups from lignin shows a decrease with increasing exposure time The differences between reference and decayed wood spectra were examined in detail using 2D correlation spectroscopy and the second derivative analysis for two exposure time periods - of 0-70 days and 70-133 days The formation of reactive species due to oxidation reactions induced by enzymes and the demethoxylation of the lignin structure was evidenced (C) 2010 Elsevier B V All rights reserved
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