Journal
MICROCHEMICAL JOURNAL
Volume 91, Issue 1, Pages 47-52Publisher
ELSEVIER
DOI: 10.1016/j.microc.2008.07.003
Keywords
Yogurt; Protein content; ATR-FTIR; Artificial neural network; Back propagation; Successive Projection Algorithm
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A method has been introduced for quantitative determination of protein content in yogurt samples based on the characteristic absorbance of protein in 1800-1500 cm(-1) spectral region by mid-FTIR spectroscopy and chemometrics. Successive Projection Algorithm (SPA) wavelength selection procedure, coupled with feed forward Back-Propagation Artificial Neural Network (BP-ANN) model was the benefited chemometric technique. Relative Error of Prediction (REP) in BP-ANN and SPA-BP-ANN methods for training set was 7.25 and 3.70 respectively. Considering the complexity of the sample, the ANN model was found to be reliable, while the proposed method is rapid and simple, without any sample preparation step. (C) 2008 Elsevier B.V. All rights reserved.
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