Journal
MICROBIOLOGY-SGM
Volume 158, Issue -, Pages 539-546Publisher
SOC GENERAL MICROBIOLOGY
DOI: 10.1099/mic.0.054403-0
Keywords
-
Categories
Funding
- The National Institute of Allergy and Infectious Disease of the National Institutes of Health [AI 19497]
- Public Health Training [T32-GM007310]
Ask authors/readers for more resources
Despite the detrimental role that endogenously generated reactive oxygen species (ROS) may play in bacteria exposed to aerobic environments, very few sources of ROS have been identified in vivo. Such studies are often precluded by the presence of efficient ROS-scavenging pathways, like those found in the aerotolerant anaerobe Bacteroides fragilis. Here we demonstrate that deletion of the genes encoding catalase (Kat), alkylhydroperoxide reductase (AhpC) and thioredoxin-dependent peroxidase (Tpx) strongly inhibits H2O2 detoxification in B. fragilis, thereby allowing for the quantification of ROS production. Exogenous fumarate significantly reduced H2O2 production in a Delta ahpC Delta kat Delta tpx B. fragilis strain, as did deletion of fumarate reductase subunit c (frdC). Deletion of frdC also increased the aerotolerance of a strain lacking superoxide dismutase, indicating that fumarate reductase is a major contributor to ROS formation in B. fragilis exposed to oxygen.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available