Journal
MICROBIOLOGY-SGM
Volume 157, Issue -, Pages 859-867Publisher
MICROBIOLOGY SOC
DOI: 10.1099/mic.0.044701-0
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Funding
- Biotechnology and Biological Sciences Research Council (BBSRC)
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Pseudomonas aeruginosa is an important pathogen in cystic fibrosis patients, and a model organism for the study of nosocomially acquired infections, biofilms and intrinsic multidrug resistance. In this study we characterize phi PA3, a new generalized transducing bacteriophage for P. aeruginosa. phi PA3 transduced chromosomal mutations between PAO1 strains, and infected multiple P. aeruginosa clinical isolates as well as the P. aeruginosa model laboratory strains PAK and PA14. Electron microscopy imaging was used to classify phi PA3 in the order Caudovirales and the family Myoviridae. The genome of phi PA3 was sequenced and found to contain 309 208 bp, the second-largest bacteriophage currently deposited in Gen Bank. The genome contains 378 ORFs and five tRNAs. Many ORF products in the phi PA3 genome are similar to proteins encoded by P. aeruginosa phage phi KZ and Pseudomonas chlororaphis phage 201 phi 2-1, and so phi PA3 was classified genetically as a member of the phi KZ-like group of phages. This is the first report of a member of this group of phages acting as a generalized transducer. Given its wide host range, high transduction efficiency and large genome size, the 'jumbo' phage phi PA3 could be a powerful tool in functional genomic analysis of diverse P. aeruginosa strains of fundamental and clinical importance.
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