Journal
MICROBIOLOGY-SGM
Volume 156, Issue -, Pages 2327-2335Publisher
MICROBIOLOGY SOC
DOI: 10.1099/mic.0.035220-0
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Funding
- Wellcome Trust
- Biotechnology & Biological Sciences Research Council
- British Society for Antimicrobial Chemotherapy
- Verein zur Forderung der angewandten Mikrobiologie
- European Community [LSHM-CT-2003-503335]
- Spanish Ministry of Education [BFU2006-04574]
- Society for General Microbiology Vacation Scholarship
- Studienstiftung des deutschen Volkes
- University of Bristol postgraduate scholarship
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We have studied the mechanism by which beta-lactam challenge leads to beta-lactamase induction in Aeromonas hydrophila through transposon-insertion mutagenesis. Disruption of the DD-carboxypeptidases/endopeptidases, penicillin-binding protein 4 or BlrY leads to elevated monomer-disaccharide-pentapeptide levels in A. hydrophila peptidoglycan and concomitant overproduction of beta-lactamase through activation of the BlrAB two-component regulatory system. During beta-lactam challenge, monomer-disaccharide-pentapeptide levels increase proportionately with beta-lactamase production and beta-lactamase induction is inhibited by vancomycin, which binds muro-pentapeptides Taken together, these data strongly suggest that the Aeromonas spp beta-lactamase regulatory sensor kinase, BlrB, responds to the concentration of monomer-disaccharide-pentapeptide in peptidoglycan
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