Journal
MICROBIOLOGY AND IMMUNOLOGY
Volume 55, Issue 2, Pages 123-129Publisher
WILEY
DOI: 10.1111/j.1348-0421.2010.00292.x
Keywords
internalin B; Listeria monocytogenes; M cell; Peyer's patches
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Funding
- [18073015]
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Listeria monocytogenes (Lm) invades the host intestine using listerial invasion proteins, internalins. The in vivo role of internalin A (InlA) and internalin B (InlB) is reported here. Intragastric (i.g.) administration and ligated loop assays with delta inlB-Lm demonstrated that a lack of InlB significantly attenuates the invasive ability of Lm into various organs. On the other hand, InlAm-Lm expressing a mutant InlA with two substitutions, S192N and Y369S, which has been reported to increase the affinity of InlA to mouse E-cadherin, resulted in little increase in intestinal infection according to both ligated loop and i.g. infection assays. Lm preferentially enters ileal Peyer's patch (PP) via M cells and delta inlB-Lm showed severely reduced ability to invade though these cells. The present results reveal the importance of InlB, which accelerates listerial invasion into M cells on ileal PPs in vivo.
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