Journal
MICROBIOLOGY AND IMMUNOLOGY
Volume 52, Issue 12, Pages 625-630Publisher
WILEY
DOI: 10.1111/j.1348-0421.2008.00079.x
Keywords
particle formation; SARS-CoV; VLP
Categories
Funding
- Ministry of Education, Science, Sports, and Culture of Japan [18780225]
- National Project on Protein Structural and Functional Analyses
- Grants-in-Aid for Scientific Research [18780225] Funding Source: KAKEN
Ask authors/readers for more resources
SARS-CoV has four major structural proteins: the N, S, M, and E proteins. To investigate the mechanism of SARS-CoV assembly, we cloned the genes encoding these four proteins into the eukaryotic expression vector pCAGGS and transfected them into 293T cells. When all four expression vectors were co-transfected VLP formed, as confirmed using electron microscopy. Using a rabbit polyclonal antibody specific to the N protein, N-protein-containing particles similar in size to the VLP were also observed by immunoelectron microscopy, indicating that the VLP contained the N protein. Co-immunoprecipitation analyses demonstrated an interaction between the N and M proteins, suggesting that N protein binds directly to M protein to be incorporated into VLP.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available