Journal
MICROBIOLOGICAL RESEARCH
Volume 169, Issue 5-6, Pages 441-452Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.micres.2013.08.005
Keywords
Reporter assay; Transcriptional regulation; Xanthomonas
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Funding
- National Science Council of Taiwan [NSC 101-2313-B-166-001-MY3]
- Changhua Christian Hospital of Taiwan [101CCH-IRP-65]
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The Gram-negative plant pathogen Xanthomonas campestris pv. campestris (Xcc) is the causative agent of black rot in crucifers, a disease that causes tremendous agricultural loss. In this study, the Xcc galE gene was characterized. Sequence and mutational analysis demonstrated that the Xcc galE encodes a UDP-galactose 4-epimerase (EC 5.1.3.2), which catalyzes the interconversion of UDP-galactose and UDPglucose. Alanine substitution of the putative catalytic residues (Ser124, Tyr147, and Lys151) of GalE caused loss of epimerase activity. Further study showed that the Xcc galE mutant had reduced biofilm formation ability. Furthermore, reporter assays revealed thatga/E transcription exhibits a distinct expression profile under different culture conditions, is subject to catabolite repression, and is positively regulated by Clp and RpfF. In addition, the galE transcription initiation site was mapped. This is the first time that UDP-galactose 4-epimerase has been characterized in the crucifer pathogen Xcc. (C) 2013 Elsevier GmbH. All rights reserved.
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