Journal
MICROBIOLOGICAL RESEARCH
Volume 168, Issue 3, Pages 125-129Publisher
ELSEVIER GMBH
DOI: 10.1016/j.micres.2012.11.004
Keywords
Bacillus subtilis subsp subtilis; Surfactin; Listeria monocytogenes; Bacteriocin-resistance
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Funding
- CIUNSa [PI 1974]
- Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT) of Argentina [PIPICTR890/06]
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Three surfactin-producing Bacillus subtilis strains, C4, M1 and G2III, previously isolated from honey and intestines from the Apis mellifera L. bee, were phylogenetically characterized at sub-species level as B. subtilis subsp. subtilis using gyrA gene sequencing. The antagonistic effect of surfactin was studied against seven different Listeria monocytogenes strains, 6 of which were resistant to bacteriocins. Surfactin showed anti-Listeria activity against all 7 strains and a dose of 0.125 mg/mL of surfactin was enough to inhibit this pathogen. Surfactin sintetized by B. subtilis subsp. subtilis C4 inhibited the pathogen in lower concentrations, 0.125 mg/mL, followed by G2I11 and M1 with 0.25 and 1 mg/mL, respectively. In particular, a dose of 0.125 mg/mL reduced the viability of L. monocytogenes 99/287 RB6, a bacteriocin-resistant strain, to 5 log orders. Surfactin assayed maintained anti-Listeria activity within a pH range of between 2 and 10, after heat treatment (boiling for 10 min and autoclaving at 121 degrees C for 15 min) and after treatment with proteolytic enzymes. These results suggest that surfactin can be used as a new tool for prevention and the control of L monocytogenes in different environments, for example, in the food industry. (C) 2012 Elsevier GmbH. All rights reserved.
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