Journal
MICROBIOLOGICAL RESEARCH
Volume 167, Issue 4, Pages 226-230Publisher
ELSEVIER GMBH
DOI: 10.1016/j.micres.2011.09.002
Keywords
Antibiotic resistance; MALDI TOF; PFGE; Vibrio; Wastewater
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The aim of this study was to discriminate 30 Vibrio strains isolated from two wastewater treatment plants from Agadir, Morocco by two molecular typing methods, pulsed-field gel electrophoresis (PFGE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Out of the 30 strains of Vibrio examined in this study, 5 isolates could not be typed by PFGE and consistently appeared as a smear on the gel. In general, high genetic biodiversity among the Vibrio strains was found regardless to the isolation source. The results of MALDI TOF analysis show a high congruence of strain grouping demonstrating the accuracy and reliability of MALDI-TOF MS. (C) 2011 Elsevier GmbH. All rights reserved.
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