Journal
MICROBIOLOGICAL RESEARCH
Volume 167, Issue 9, Pages 564-570Publisher
ELSEVIER GMBH
DOI: 10.1016/j.micres.2011.11.006
Keywords
Diarrheagenic E. coli; Multiplex PCR
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Funding
- Swedish Research Council
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Establishment of a simple and rapid multiplex PCR system for identification of the main diarrheagenic E. coli categories, including enteroaggregative E. coli. enterotoxigenic E. coli, enteropathogenic E. coli, and enterohemorrhagic E. coli, is described. This two-step multiplex PCR system allows the identification by targeting CVD432, LT, STh, Sip, Eae, Bfp, Stx1, and Stx2. By applying the developed multiplex PCR system, categorization of E. coli isolates isolated from stool samples of infants with diarrhea into the main diarrheagenic E. coli categories is also shown. (C) 2011 Elsevier GmbH. All rights reserved.
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