Journal
MICROBIOLOGICAL RESEARCH
Volume 166, Issue 6, Pages 475-483Publisher
ELSEVIER GMBH
DOI: 10.1016/j.micres.2010.09.002
Keywords
Armillariella tabescens; Aflatoxin-oxidase (AFO); Hydrophobic interaction chromatography (HIC); Immobilized metal ion affinity chromatography (IMAC); ESI-MS/MS
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Funding
- National High Technology Research and Development Program of China (863 Program) [2002AA213011, 2005AA213010]
- National Natural Science Foundation of China [30270043]
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Armillariella tabescens, a Chinese edible and medicinal fungus, whose multienzyme exist ability of AFB(1)-converting, and ADTZ (aflatoxin-detoxizyme) had previously purified from the A. tabescens multienzyme monitored by AFB(1) conversion. However, the enzyme now confirmed an oxidase and renamed aflatoxin-oxidase (AFO). In this paper, AFO was purified by an economical and practical three-step procedure monitored by AFB(1) conversion. And ESI-MS/MS analysis was done for identification of AFO. The following database searching (Protein Blast on NCBI) results did not show any homologous oxidase protein, which implied that AFO was mostly a new oxidase differing from other reported aflatoxin-converting enzymes such as fungal laccase and horse radish peroxidase. HPTLC analysis of the purified AFO activity suggested that the enzyme reacted at the bisfuran ring of AFB(1) which was the key toxic structure. Therefore, all these investigations implied a new choice for biodegradation of aflatoxin in foods and feeds with the practical application of AFO. (C) 2010 Elsevier GmbH. All rights reserved.
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