Journal
MICROBIOLOGICAL RESEARCH
Volume 164, Issue 6, Pages 650-657Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.micres.2008.12.002
Keywords
Cellulase; Metagenomic library; Cloning; Uncultured microorganism
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Funding
- National Natural Science Program of China [30770063]
- National Basic Research Program of China [2004CB719702]
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Four different environmental DNA libraries were prepared from microbial consortium collected from forest soil, dung of elephant, cow rumen and rotted tree. Seven independent clones specifying cellulase activities (five endo-beta-1,4-glucanases and two beta-glucosidases) were isolated and identified. Sequence analysis of the retrieved genes revealed that the encoded products of these cellulase genes shared less than 50% identities and 70% similarities to cellulases in the databases. Domain analysis predicted that four endo-beta-1,4-glucanases conform to glycolsyl hydrolase family 5 (GHF 5) and one endo-beta-1,4-glucanase to glycosyl hydrolase family 9 (GHF 9), while both beta-glucosidases belong to glycolsyl hydrolase family 3 (GHF 3). Further sequence analysis indicated that although a solid affiliation could be made for the two endo-beta-1,4-glucanases to the typical ruminal microbe Prevotella ruminicola, the rest formed deep-branched lineages with no close relatives. The revelation of the phylogenetic novelty provided a snapshot on the great diversity of cellulases in these natural environments. (C) 2008 Elsevier GmbH. All rights reserved.
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