Journal
MICROBIOLOGICAL RESEARCH
Volume 164, Issue 2, Pages 174-179Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.micres.2007.01.001
Keywords
Type A avian influenza virus infections; H9N2 subtype; Multiplex RT-PCR
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Funding
- Ministry of Science, Technology and Innovation, Malaysia [01-02-04009-BTK/ER/38]
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Subtype-specific multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed to simultaneously detect three subtypes (H5, H7 and H9) of avian influenza virus (AIV) type A. The sensitivity of the multiplex RT-PCR was evaluated and compared to that of RT-PCR-enzyme-linked immunosorbent assay (ELISA) and conventional RT-PCR. While the sensitivity of the multiplex RT-PCR is as sensitive as the conventional RT-PCR, it is 10 times less sensitive than RT-PCR-ELISA. The multiplex RT-PCR is also as sensitive as the virus isolation method in detecting H9N2 from tracheal samples collected at day 3 and 5 post inoculation. Hence, the developed multiplex RT-PCR assay is a rapid, sensitive and specific assay for detecting of AN subtypes. (C) 2007 Elsevier GmbH. All rights reserved.
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