Journal
MICROBIAL PATHOGENESIS
Volume 125, Issue -, Pages 281-289Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2018.09.023
Keywords
Porcine reproductive and respiratory syndrome virus; 2 ',5 '-oligoadenylate synthetase; Replication; Porcine alveolar macrophages; RNase L; Bioinformatics
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Funding
- Scientific Research Foundation of the Programs for Science and Technology Development of Henan Province, China [162102110033, 182102110083]
- Doctoral Startup Fund for Henan University of Animal Husbandry and Economy
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Porcine 2',5'-oligoadenylate synthetase-like protein is an essential antiviral protein induced by interferons; however, its bioinformatics, genetic characteristics and immunological characteristics related to porcine reproductive and respiratory syndrome virus are still unknown. In this study, porcine 2',5'-oligoadenylate synthetase-like protein was cloned, and various attributes were predicted by bioinformatics analysis. Through RNAi depletion and overexpression methods, it was determined that porcine OASL not only inhibits porcine reproductive and respiratory virus replication but also activates interferon-beta production and the interferon-beta promoter, promoting the expression of interferon-beta mRNA. Through the depletion of different amino acids at the N and C termini, the antiviral activity and promoting the activity of interferon beta were evaluated. The results demonstrated that 31-60 amino acids at the N terminus were critical for virus replication. This study laid a theoretical foundation for exploring the characteristics of the porcine 2',5'-oligoadenylate synthetase-like protein and suggested a new strategy for the prevention and control of porcine reproductive and respiratory syndrome virus and investigation of the therapeutic mechanism of this protein.
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