Journal
MICROBIAL PATHOGENESIS
Volume 55, Issue -, Pages 32-38Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2012.09.010
Keywords
F18+E. coli; Flagella; Adherence; Adhesin
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Funding
- Chinese National Science Foundation Grant [31072136, 31270171, 30771603]
- Jiangsu High Education Key Basic Science Foundation [08KJA230002]
- Genetically Modified Organisms Technology Major Project of China [2009ZX08006-004B]
- Priority Acdemic Program of Development Jiangsu High Education Institution, Program for ChangJiang Scholars and Innovative Research Team In university PCSIRT [IRT0978]
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F18 fimbriae and toxins produced by F18 fimbriae-carrying Escherichia coli (E. coli) strains are known virulence factors responsible for post-weaning diarrhea (PWD) and edema disease (ED). In this study, we showed that fliC isogenic mutants constructed in two reference wild-type F18 fimbriae (F18+) E. coli were markedly impaired in adherence in vitro cell models (p < 0.05). Flagella purified from F18+ E. coli could directly bind to cultured piglet epithelial cells and block adherence of F18+ E. coli to cells when pre-incubated. In addition, the F18+ E. coli fliC deletion mutants up-regulated the expression of type I fimbriae produced by F18+ E. coli strains. These results demonstrated that expression of flagella is essential for the adherence of F18+ E. coli in vitro. (c) 2012 Elsevier Ltd. All rights reserved.
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