Journal
MICROBIAL ECOLOGY
Volume 58, Issue 3, Pages 621-631Publisher
SPRINGER
DOI: 10.1007/s00248-009-9516-x
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Funding
- Academy of Finland [106208, 123725, 105860, 120089]
- Emerging Frontiers
- Direct For Biological Sciences [0732956] Funding Source: National Science Foundation
- Academy of Finland (AKA) [120089, 106208, 123725, 106208, 120089, 105860, 123725, 105860] Funding Source: Academy of Finland (AKA)
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The effect of freeze-thaw (FT) cycles on Arctic tundra soil bacterial community was studied in laboratory microcosms. FT-induced changes to the bacterial community were followed over a 60-day period by terminal restriction fragment length polymorphism (T-RFLP) profiles of amplified 16S rRNA genes and reverse transcribed 16S rRNA. The main phylotypes of the active, RNA-derived bacterial community were identified using clone analysis. Non-metric multidimensional scaling ordination of the T-RFLP profiles indicated some shifts in the bacterial communities after three to five FT cycles at -2, -5, and -10A degrees C as analyzed both from the DNA and rRNA. The dominating T-RFLP peaks remained the same, however, and only slight variation was generally detected in the relative abundance of the main T-RF sizes of either DNA or rRNA. T-RFLP analysis coupled to clone analysis of reverse transcribed 16S rRNA indicated that the initial soil was dominated by members of Bacteroidetes, Acidobacteria, Alpha-, Beta-, and Gammaproteobacteria. The most notable change in the rRNA-derived bacterial community was a decrease in the relative abundance of a Betaproteobacteria-related phylotype after the FT cycles. This phylotype decreased, however, also in the control soil incubated at constant +5A degrees C suggesting that the decrease was not directly related to FT sensitivity. The results indicate that FT caused only minor changes in the bacterial community structure.
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