Journal
MICROBIAL CELL FACTORIES
Volume 13, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1475-2859-13-63
Keywords
Bacillus species; Genetic engineering strategies; Counter-selection marker; Operator-repressor system; Toxin gene; Site-specific recombination
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Funding
- State Key Development Program for Basic Research of China (973 Program) [2013CB733600]
- National Nature Science Foundation of China [31200036, 31370089]
- Key Projects in the Tianjin Science & Technology Pillar Program [12ZCZDSY12700, 11ZCZDSY08500, 11ZCZDSY08400]
- K. C. Wong Education Foundation, Hong Kong
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The complete sequencing and annotation of the genomes of industrially-important Bacillus species has enhanced our understanding of their properties, and allowed advances in genetic manipulations in other Bacillus species. Post-genomic studies require simple and highly efficient tools to enable genetic manipulation. Here, we summarize the recent progress in genetic engineering strategies for Bacillus species. We review the available genetic tools that have been developed in Bacillus species, as well as methods developed in other species that may also be applicable in Bacillus. Furthermore, we address the limitations and challenges of the existing methods, and discuss the future research prospects in developing novel and useful tools for genetic modification of Bacillus species.
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