4.7 Article

Modification of genetic regulation of a heterologous chitosanase gene in Streptomyces lividans TK24 leads to chitosanase production in the absence of chitosan

Journal

MICROBIAL CELL FACTORIES
Volume 10, Issue -, Pages -

Publisher

BIOMED CENTRAL LTD
DOI: 10.1186/1475-2859-10-7

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Funding

  1. Natural Science and Engineering Research Council (NSERC) of Canada
  2. NSERC
  3. Fonds quebecois de recherche sur la nature et les technologies

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Background: Chitosanases are enzymes hydrolysing chitosan, a beta-1,4 linked D-glucosamine bio-polymer. Chitosan oligosaccharides have numerous emerging applications and chitosanases can be used for industrial enzymatic hydrolysis of chitosan. These extracellular enzymes, produced by many organisms including fungi and bacteria, are well studied at the biochemical and enzymatic level but very few works were dedicated to the regulation of their gene expression. This is the first study on the genetic regulation of a heterologous chitosanase gene (csnN106) in Streptomyces lividans. Results: Two S. lividans strains were used for induction experiments: the wild type strain and its mutant (Delta csnR), harbouring an in-frame deletion of the csnR gene, encoding a negative transcriptional regulator. Comparison of chitosanase levels in various media indicated that CsnR regulates negatively the expression of the heterologous chitosanase gene csnN106. Using the Delta csnR host and a mutated csnN106 gene with a modified transcription operator, substantial levels of chitosanase could be produced in the absence of chitosan, using inexpensive medium components. Furthermore, chitosanase production was of higher quality as lower levels of extracellular protease and protein contaminants were observed. Conclusions: This new chitosanase production system is of interest for biotechnology as only common media components are used and enzyme of high degree of purity is obtained directly in the culture supernatant.

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