Journal
MICROBES AND INFECTION
Volume 15, Issue 4, Pages 319-328Publisher
ELSEVIER
DOI: 10.1016/j.micinf.2013.01.005
Keywords
HIV-1; HIV-1mt; Pol-IN; Env-gp120; Adaptive mutation; Macaque cells
Categories
Funding
- Ministry of Health, Labour and Welfare of Japan [H23-003]
- Grants-in-Aid for Scientific Research [23390111, 24659208, 23590541] Funding Source: KAKEN
Ask authors/readers for more resources
Fundamental property of viruses is to rapidly adapt themselves under changing conditions of virus replication. Using HIV-1 derivatives that poorly replicate in macaque cells as model viruses, we studied here mechanisms for promoting viral replication in non-natural host cells. We found that the HIV-1s could evolve to grow better in both macaque and human cells by the continuous culture in macaque lymphocyte cell lines. Notably, only several mutations at defined sites of the Pol-integrase and/or the Env-gp120 reproducibly appeared in repeated adaptation experiments and were sufficient to cause the phenotypic change. Meanwhile, no amino acid changes to enhance viral replication in macaque cells were found in interaction sites for the known anti-retroviral proteins. These findings disclose a hitherto unappreciated evolutionary pathway to augment HIV-1 replication in primate cells, where tuning of viral interactions with positive rather than negative factors for replication can play a dominant role. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available