Journal
MICROBES AND INFECTION
Volume 14, Issue 11, Pages 951-958Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micinf.2012.04.002
Keywords
Biofilms; Pseudomonas aeruginosa; Tumor; Animal model
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Funding
- Deutsche Krebshilfe
- German Ministry of Education and Research (BMBF)
- German Research Council (DFG)
- Helmholtz Association via HIRSIB
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The ability of opportunistic bacterial pathogens to grow in biofilms is decisive in the pathogenesis of chronic infectious diseases. Growth within biofilms does not only protect the bacteria against the host immune system but also from the killing by antimicrobial agents. Here, we introduce a mouse model in which intravenously administered planktonic Pseudomonas aeruginosa bacteria are enriched in transplantable subcutaneous mouse tumors. Electron microscopy images provide evidence that such bacteria reside in the tumor tissue within biofilm structures. Immunohistology furthermore demonstrated that infection of the tumor tissue elicits a host response characterized by strong neutrophilic influx. Interestingly, the biofilm defective PA14 pysA transposon mutant formed less biofilm in vivo and was more susceptible to clearance by intravenous ciprofloxacin treatment as compared to the wild-type control. In conclusion, we have established an experimentally tractable model that may serve to identify novel bacterial and host factors important for in vivo biofilm formation and to re-evaluate bactericidal and anti-biofilm effects of currently used and novel antibacterial compounds. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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