EXO70 protein influences dengue virus secretion

The involvement of host proteins in assisting the exocytosis of flaviviruses is largely unknown. In this study, we aimed to investigate if dengue virus (DENV) utilizes the exocyst components to aid the exocytosis of virus particles. This study identified that EXO70 protein, a member of the exocyst complex influenced DENV infection. Dengue virus production was significantly attenuated in EXO70 knock-down cells. EXO70 did not influence viral transcription and translation. It influenced virus egression/secretion from DENV-infected cells. We also showed that EXO70 expression was up-regulated from 18 h post-infection following DENV infection. Although the envelope protein of DENV influenced EXO70 expression, the co-expression of pre-membrane and envelope proteins significantly increased the expression levels of EXO70 during DENV infection. When pre-membrane protein was expressed alone, there was no significant difference in the expression levels of EXO70. This indicated that the presence of pre-membrane protein might help in the proper folding of envelope protein. Increased expression levels of EXO70 might help in the exocytosis process of virus or subviral particles. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.