Journal
MICROBES AND ENVIRONMENTS
Volume 28, Issue 2, Pages 236-243Publisher
JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
DOI: 10.1264/jsme2.ME12209
Keywords
continuous feeding bioreactor; nitrification; Nitrospira; uncultured bacteria
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Funding
- Industrial Technology Research Grant Program from the New Energy and Industrial Technology Development Organization of Japan [05A07508a]
- Sasagawa Scientific Research Grant from The Japan Science Society
- Waseda University [2011B-200, 2012B-171]
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Nitrification is an important step in nitrogen removal in biological wastewater treatment processes. Recently, Nitrospira have been recognized as the numerically dominant nitrite-oxidizing bacterial genus primarily responsible for the second step of aerobic nitrification; however, Nitrospira usually resist cultivation under laboratory conditions and only one species enriched from activated sludge has been described. In this study, a novel enrichment method for Nitrospira was successfully developed using continuous feeding bioreactors. By controlling nitrite concentrations strictly in the bioreactor at low levels below 10 mg-N L-1, coexisting members of sublineages I and II of the genus Nitrospira were enriched selectively. The maximum ratios of sublineages I and II to total microbial cells achieved 88.3% and 53.8%, respectively. This enrichment method is potentially applicable to other uncultured Nitrospira.
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