Journal
METHODS
Volume 68, Issue 1, Pages 273-279Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2014.03.022
Keywords
Adult Drosophila midgut; Stem cell; Lineage tracing; Environmental challenge
Funding
- NIDDK NIH HHS [P30 DK052574, R01 DK095871] Funding Source: Medline
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The gastrointestinal (GI) tract harbors an essential barrier epithelium that separates an organism from its changing external environment. As such, the gut epithelium is a fascinating nexus of stem cell biology, immunology and physiology. Investigators have sought to mine this rich interface for new biological and mechanistic insights. Many of the powerful genetic approaches developed in Drosophila have proven effective in the study of the gut. The goal of this article is to present a method for dissecting, immunostaining and mounting samples of the adult Drosophila Cl tract. This protocol combines readily with techniques to label cell lineages and/or challenge the system with environmental perturbations, which are briefly discussed. (C) 2014 Published by Elsevier Inc.
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