Journal
METHODS
Volume 69, Issue 2, Pages 137-141Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2014.03.026
Keywords
ZFNs; TALENs; DNA repair; Gene targeting; NHEJ; Homologous recombination
Funding
- United States National Institutes of Health [R01 GM078571]
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Genome engineering with targetable nucleases depends on cellular pathways of DNA repair after target cleavage. Knowledge of how those pathways work, their requirements and their active factors, can guide experimental design and improve outcomes. While many aspects of both homologous recombination (HR) and nonhomologous end joining (NHEJ) are shared by a broad range of cells and organisms, some features are specific to individual situations. This article reviews the influence of repair mechanisms on the results of gene targeting experiments, with an emphasis on lessons learned from experiments with Drosophila. (C) 2014 Elsevier Inc. All rights reserved.
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