Journal
METHODS
Volume 68, Issue 3, Pages 465-475Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2014.04.002
Keywords
Aging; Lifespan; C. elegans; Stress assay; High throughput; Longevity
Funding
- Pennsylvania Department of Health
- Health Research Formula Funds
- Ellison Medical Foundation
- Children's Hospital of Pittsburgh
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Since the discovery of single gene mutations that double its lifespan, the nematode Caenorhabditis elegans has provided remarkable insights into the biology of aging. The precisely measurable lifespan of worms has proven to be an efficient tool to assess the impact of various genetic, physiological and environmental factors on organismal aging. In this article, we describe methods to set up and monitor experiments to determine worm lifespan. We include procedures used for classical, small-scale lifespan assays that are generally performed on solid media, and review recent advances in high-throughput, automated longevity experiments conducted in liquid culture and microfiuidic devices. In addition, tools that help analyze this data to obtain survival statistics are summarized, and C elegans strains that offer particular advantages for lifespan studies are listed. (C) 2014 Elsevier Inc. All rights reserved.
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