Journal
METHODS
Volume 68, Issue 2, Pages 317-324Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2014.02.012
Keywords
Stable isotope; NanoSIMS; Atomic force microscopy; Backscattered electron imaging; Correlative analysis
Funding
- China Scholarship Council
- NIH [HL090553, HL087228]
- Leducq Transatlantic Network Grant [12CVD04]
- American Heart Association, Western States Affiliate
- United Kingdom's Department of Business, Innovation and Skills
- European Community's Seventh Framework Programme, ERA-NET Plus [217257]
- Cancer Research UK [11359] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [GR/T19797/01, EP/E036384/1] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0611-10163] Funding Source: researchfish
- EPSRC [EP/E036384/1] Funding Source: UKRI
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Stable isotopes are ideal labels for studying biological processes because they have little or no effect on the biochemical properties of target molecules. The NanoSIMS is a tool that can image the distribution of stable isotope labels with up to 50 rim spatial resolution and with good quantitation. This combination of features has enabled several groups to undertake significant experiments on biological problems in the last decade. Combining the NanoSIMS with other imaging techniques also enables us to obtain not only chemical information but also the structural information needed to understand biological processes. This article describes the methodologies that we have developed to correlate atomic force microscopy and backscattered electron imaging with NanoSIMS experiments to illustrate the imaging of stable isotopes at molecular, cellular, and tissue scales. Our studies make it possible to address 3 biological problems: (1) the interaction of antimicrobial peptides with membranes; (2) glutamine metabolism in cancer cells; and (3) lipoprotein interactions in different tissues. (C) 2014 Elsevier Inc. All rights reserved.
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